FPrime Recommended Article: Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited. Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited The Michaelis-Menten equation accurately describes the bulk behavior of. Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited. Nature Chemical Biology, 2(2), DOI: /nchembio
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I am an author of this article. Showing of 48 references. Neither of the above. We monitored long time traces of enzymatic turnovers for individual beta-galactosidase molecules by detecting one fluorescent product at equqtion time. Register Already registered with FPrime? University of Groningen staff: No warranty is given about the accuracy of the copy.
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Illuminating single molecules in condensed matter. By posting or uploading Material you warrant and represent that: Here we tested the Refisited equation at the single-molecule level. Citations Publications citing this paper. Rate processes with dynamical disorder: The classic Michaelis-Menten mechanism provides a highly satisfactory description of catalytic activities for large ensembles of enzyme molecules.
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High Spatiotemporal-Resolution Magnetic Tweezers: Such memory lasts for decades of timescales ranging from milliseconds midhaelis seconds owing to the presence of interconverting conformers with broadly distributed lifetimes.
Recommendations Abstract Comments You have reached your article limit. Consider the following examples, enzhme note that this is not an exhaustive list: The structure of E. Quantitative interpretation of the randomness in single enzyme turnover times.
Here we tested the Michaelis-Menten equation at the single-molecule level. Hans-Philipp LerchRudolf W. A molecular memory phenomenon arises at high substrate concentrations, characterized by clusters of turnover events separated by periods of low activity.
Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited.
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Classified as close New Finding 2. This entry form currently does not support special characters. Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited ‘ Nature Fluctuatijg Biologyvol 2, no. We proved that the Michaelis-Menten equation still holds even for a fluctuating single enzyme, but bears a different microscopic interpretation. University of Groningen, Zernike Institute for Advanced Materials PY – Y1 – N2 – Enzymes are biological catalysts vital to life processes and have attracted century-long investigation.
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The classic Michaelis-Menten mechanism provides a highly satisfactory description of catalytic activities for large ensembles of enzyme molecules. A summary of the content will be automatically included. Institutional access Recommend FPrime to your librarian or information manager to request an extended free trial for all users at your institution.