The system uses the procedures described in AOCS Ce , which is based on triglyceride hydrolysis and reaction with BF3. After methylation, FAMEs are. 1Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Afyonkarahisar, Turkey. 2Akdeniz University, Faculty. AOCS Official Method Ce, “Preparations of Methyl Esters of Fatty Acids,” , pp . has been cited by the following article: TITLE: Trans Fatty Acid Content.

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This was done to assure aos any observed FAME recovery differences were a result of incomplete conversion and not due to incomplete hexane extraction, with glyceryl triheptadecanoate used to monitor the completeness of both the esterification reaction and the ease of extraction of the resulting FAMEs, while the methyltridecanoate was used to monitor only the effectiveness of the hexane extraction step alcs FAME recovery.

Close mobile search navigation Article navigation. Also, the high temperatures that are required to elute and detect high-boiling species can lead to analyte degradation before detection 4.

Place the column under a carrier gas flow e. Preparation of fatty acid methyl esters for gas-chromatographic analysis of lipids in biological materials. Assembly and conditioning of the column— a Observe the normal precautions for assembling capillary columns—arrangement of the column in the oven supportchoice and assembly of joints leak tightnesspositioning of the ends of the column in the injector and the detector reduction of dead spaces. The coating should be 0.

It will be n e c e s s a ry to optimize the operating conditions by acting on this parameter or more simply on the deadspace of the columnaccording to whether one wishes to improve the separations or to make a rapid analysis.

To demonstrate method effectiveness on a complex biological oil, a well-characterized sesame oil standard was reacted under the optimized selective esterification conditions 2. Abstract A method for selectively determining both free fatty acids FFA and triacylglycerides TAGs in biological oils was investigated and optimized using gas chromatography after esterification of the target species to their corresponding fatty acid methyl esters FAMEs.


The chromatographic parameters are described in the following. Related articles in Web of Science Google Scholar. Syringe—max capacity of 10? It should be compatible with the apparatus used. Composition of sesame oil samples reacted under selective esterification conditions and full transesterification conditions.

Fatty Acid Methyl Esters – SampleQ

Furthermore, only trace amounts of C A unique double-spike methodology was also employed in which oil samples were spiked with a TAG conversion standard glyceryl triheptadecanoate and a FAME extraction recovery standard methyltridecanoate. Rapid and accurate calculations can be performed with the help of an electronic i n t egrator or calculat o r, but it must give a linear response with adequate sensitivity and the correction for deviation of the baseline must be satisfactory. Either helium or nitrogen may be suitable as a carrier gas, but these may increase helium elution times with respect aocss hydrogen.

To our knowledge, this is the first instance of a double-spike experimental protocol being used to separately evaluate both the conversion reaction and the extraction step, while at the same time quantifying both using FAMEs. The transesterification process 2-666 FAMEs, which behave more ideally in GC analyses at lower temperatures than their parent species, and also allow for surrogate calibration species to be used.

AOCS Official Method Ce 2-66 Preparation of Methyl Esters of Fatty Acids

Sampling and analysis of commercial fats and oils, 6th edition. Citing articles via Web of Science 4.

The critical importance of identifying sources for renewable fuel has led to the pursuit of oil-producing crops and feedstocks, with increasing pressure to trend away from food-based materials and toward non-edible sources.

Selection of optimum operating conditions—The prope rties of effi c i e n cy and perm e ability of cap i 22-66 l a ry columns mean that the separation between constituents and duration of analysis are largely dependent on the flow rate of the carrier gas in the column.

An external calibration curve was prepared from pure component FFA calibration standards whose concentrations ranged from 0. Aosc optimized c demonstrated that it is possible to use esterification reactions to selectively determine the free acid content, total lipid content, and hence, glyceride content in biological oils. This protocol also allows gas chromatography analysis of FAMEs as a more ideal analyte than glyceride species in their native state.


Aaocs methods and recommended practices of the AOCS: Lipid solvent systems are not equivalent for analysis of lipid classes in the microeukaryotic green alga, chlorella. Single and mixed-component solutions of pure fatty acids and triglycerides, as well as a sesame oil sample to simulate a complex biological oil, were used to optimize the methodologies.

免费下载 AOCS Official Method Ce Preparation of Methyl Esters of Fatty Acids – WDFXW文档分享网

O p e rating the oven by linear heating increases the speed of fatty acid elution but does not improve or reduce the resolution of the gas chromatograms. The vial oacs capped and placed in a pre-equilibrated bath at the desired temperature for a known amount of time.

A sesame oil sample was used to demonstrate the effectiveness of the method on a 2-6 material. Moreover, by tracking just the concentration of oleic acid itself, 2-6 method was able to effectively recover the 0. HCl was chosen as acid catalyst to provide a simple monoprotic acid as opposed to other potential acid catalysts such as sulfuric acid and phosphoric acid.

A typical reaction for pure component testing is outlined as follows: Few, if any, standard methods exist for determining algae lipid content. In the case of sesame oil and its reaction products, the unknown peaks were identified by class [monoacylglyceride MAGdiacylglyceride DAGand TAG] by a combination of retention time matching to known standards and mass spectrometry spectral library matching.

The method used acid catalyzed esterification in methanolic solutions under conditions of varying severity to achieve complete conversion of more reactive FFAs while preserving the concentration of TAGs. Carrier gas—either helium or hydrogen see Notes, 3. Hydrochloric acid trace metal grade was purchased from Fisher Scientific and used as received. It should be of the split-type or on-column see Notes, 1.